Detection of anticardiolipin antibodies in culture supernatants

Objective: We have attempted to devise a method for measuring the levels of anticardiolipin antibodies produced in in vitro culture of peripheral bloo d mononuclear cells isolated from patients with systemic lupus erythematosu s (SLE), with or without circulating anticardiolipin antibodies, patients w ith the primary antiphosholipid syndrome (PAPS) and normal controls. Methods: peripheral blood mononuclear cells were isolated and cultured for up to six days, in the presence or absence of added cytokines in the cultur e medium. Supernatants harvested after culture were tested by ELISA for the presence of anticardiolipin antibodies. Results and Conclusion: Despite variation of the culture conditions and of the cell numbers and populations used it was found that accurately measurea ble levels of anticardiolipin antibodies could not be detected reliably in any of the culture supernatants. Ir is concluded that alternative methods o f measurement of antibody production need to be explored.