Cp. Ordahl et al., The dermomyotome dorsomedial lip drives growth and morphogenesis of both the primary myotome and dermomyotome epithelium, DEVELOPMENT, 128(10), 2001, pp. 1731-1744
The cellular and molecular mechanisms that govern early muscle patterning i
n vertebrate development are unknown. The earliest skeletal muscle to organ
ize, the primary myotome of the epaxial domain, is a thin sheet of muscle t
issue that expands in each somite segment in a lateral-to-medial direction
in concert with the overlying dermomyotome epithelium. Several mutually con
tradictory models have been proposed to explain how myotome precursor cells
, which are known to reside within the dermomyotome, translocate to the sub
jacent myotome layer to form this first segmented muscle tissue of the body
. Using experimental embryology to discriminate among these models, we show
here that ablation of the dorsomedial lip (DML) of the dermomyotome epithe
lium blocks further primary myotome growth while ablation of other dermomyo
tome regions does not, Myotome growth and morphogenesis can be restored in
a DML-ablated somite of a host embryo by transplantation of a second DML fr
om a donor embryo, Chick-quail marking experiments show that new myotome ce
lls in such recombinant somites are derived from the donor DML and that cel
ls from other regions of the somite are neither present nor required, In ad
dition to the myotome, the transplanted DML also gives rise to the dermomyo
tome epithelium overlying the new myotome growth region and from which the
mesenchymal dermatome will later emerge, These results demonstrate that the
DML is a cellular growth engine that is both necessary and sufficient to d
rive the growth and morphogenesis of the primary myotome and simultaneously
drive that of the dermomyotome, an epithelium containing muscle, dermis an
d possibly other potentialities.