An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate

Authors
Teotia, S Khare, SK Gupta, MN
Citation
S. Teotia et al., An efficient purification process for sweet potato beta-amylase by affinity precipitation with alginate, ENZYME MICR, 28(9-10), 2001, pp. 792-795
Citations number
23
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
ENZYME AND MICROBIAL TECHNOLOGY
ISSN journal
01410229 → ACNP
Volume
28
Issue
9-10
Year of publication
2001
Pages
792 - 795
Database
ISI
SICI code
0141-0229(20010607)28:9-10<792:AEPPFS>2.0.ZU;2-O
Abstract
beta -amylases are used in production of maltose syrup. It is shown that sw eet potato beta -amylase can be purified by affinity precipitation with alg inate with 80% activity yield and 44 fold purification. SDS-PAGE of the pur ified protein showed a single band and a subunit weight of 50 kDa. Prelimin ary data with soybean and barley enzymes indicate that this may be a genera l method for purification of beta -amylases. (C) 2001 Elsevier Science Inc. All rights reserved.