Perinuclear mRNA localisation by vimentin 3 '-untranslated region requiresa 100 nucleotide sequence and intermediate filaments

The role of the vimentin 3 ' -untranslated region (3 ' -UTR) in mRNA locali sation was studied in cells transfected with a reporter sequence linked to subregions of the 3 ' -UTR, Tn situ hybridisation showed that nucleotides 3 7-137, including a previously identified protein-binding domain, mere suffi cient to localise transcripts to perinuclear cytoplasm. Transfection of two SW13 cell lines that do and do not express vimentin showed that perinuclea r localisation due to either the vimentin or c-myc 3 ' -UTR requires interm ediate filaments, The data suggest that both a specific protein-binding reg ion of the vimentin 3 ' -UTR and intermediate filaments themselves are requ ired to determine the site of vimentin synthesis. (C) 2001 Federation of Eu ropean Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.