M. Islam et Ra. Akhtar, Upregulation of phospholipase C gamma 1 activity during EGF-induced proliferation of corneal epithelial cells: Effect of phosphoinositide-3 kinase, INV OPHTH V, 42(7), 2001, pp. 1472-1478
PURPOSE. Previously, the authors showed that epidermal growth factor (EGF)
stimulates phospholipase C gamma1 (PLC gamma1) and phosphoinositide-3 kinas
e (PI3K) activities in confluent rabbit corneal epithelial cells (RCECs). T
he purpose of this study was to investigate whether PLC gamma1 activity is
upregulated during EGF-induced proliferation of RCECs and to determine whet
her there is any cross-talk between PLC gamma1 and PI3K in these cells.
METHODS. Simian virus (SV)40-immortalized RCECs were cultured in the presen
ce and absence of EGF and other agents. At prescribed time intervals, the c
ultures were terminated and the cells counted. PLC gamma1 activity in intac
t cells was assessed by measuring the production of [H-3]IP, in [H-3]myoino
sitol-labeled cells. The in vitro enzyme activity was assayed using immunop
recipitated PLC gamma1 and [H-3]PI(4,5)P-2 as substrate. [H-3]IP3 the produ
ct of PLC gamma1, was analyzed by anion-exchange chromatography. The change
s in protein content and level of phosphorylation of PLC gamma1 were determ
ined by Western immunoblot analysis, with the appropriate antibodies.
RESULTS. Addition of EGF (50 ng/ml) caused a time-dependent increase in pro
liferation of RCECs. The effect of EGF peaked at approximately 36 hours. Un
der the same experimental conditions, EGF stimulated PLC gamma1 activity wi
th a time course similar to that of cell proliferation. Data from Western i
mmunoblot analysis revealed that the EGP-stimulated PLC gamma1 activity was
due to increased synthesis of the enzyme. Furthermore, during cell prolife
ration, tyrosine phosphorylation of PLC gamma1 increased in a time-dependen
t manner that corresponded closely with the expression of PLC gamma1. EGF e
xerted its effects both on cell proliferation and PLC gamma1 activation in
a dose-dependent manner. Treatment of the cells with U-73122, a PLC inhibit
or, or myr-GLYRKAMRLRY, a myristoylated PLC gamma1 inhibitor peptide, cause
d attenuation of both the EGF-stimulated cell proliferation and PLC gamma1
activity. Treatment of the cells with the PI3K inhibitors, wortmannin or LY
294002, caused inhibition of both EGF-stimulated cell proliferation and PLC
gamma1 activation. Addition of PI(3,4,5)P-3 to the in vitro PLC gamma1 ass
ay mixture stimulated the enzyme activity in a dose-dependent manner.
CONCLUSIONS. The data suggest a positive correlation between EGF-stimulated
PLC gamma1 activation and cell proliferation in RCECs. The EGF-stimulated
PLC gamma1 activity was mirrored by increased synthesis and tyrosine phosph
orylation of the enzyme. The data also show that PLC gamma1 activation and
cell proliferation were inhibited by PI3K inhibitors, suggesting a role for
PI3K in EGF-stimulated proliferation of corneal epithelial cells.