Aj. Hietaranta et al., Water immersion stress prevents caerulein-induced pancreatic acinar cell NF-kappa B activation by attenuating caerulein-induced intracellular Ca2+ changes, J BIOL CHEM, 276(22), 2001, pp. 18742-18747
Prior stress ameliorates caerulein-induced pancreatitis in rats. NF-kappaB
is a proinflammatory transcription factor activated during caerulein pancre
atitis. However, the effects of prior stress on pancreatic NF-kappaB activa
tion are unknown. In the current study, the effect of prior water immersion
stress on caerulein and tumor necrosis factor-alpha (TNF-alpha)-induced NF
-kappaB activation in the pancreas was evaluated. Water immersion of rats f
or up to 6 h prevents supramaximal caerulein-induced pancreatic I kappaB-al
pha degradation and NF-kappaB activation in vivo, NF-kappaB activity is als
o inhibited in vitro in pancreatic acini prepared from water-immersed anima
ls. TNF alpha -induced NF-kappaB activation in pancreas or in pancreatic ac
ini is unaffected by prior water immersion. Chelation of intracellular Ca2 by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate/acetoxymethyl este
r has similar effects to water immersion in preventing caerulein but not TN
F-alpha -induced NF-kappaB activation in pancreas. Both the spike response
and the sustained rise in [Ca2+], in response to supramaximal caerulein sti
mulation are reduced markedly in acini prepared from water-immersed animals
as compared with normal animals. Our findings indicate that, in addition t
o Ca2+-dependent mechanisms, Ca2+-independent signaling events also may lea
d to NF-kappaB activation in pancreatic acinar cells. Water immersion stres
s prevents supramaximal caerulein-induced NF-kappaB activation in pancreas
in vivo and in vitro by affecting intracellular Ca2+ homeostasis.