Polyaniline-dacron composite as solid phase in enzyme linked immunosorbentassay for Yersinia pestis antibody detection

Polyaniline (PANI) was chemically synthesized on a dacron disk surface and an antigen (F1 fraction) obtained from Yersinia pestis was covalently fixed onto this composite via glutaraldehyde. The enzyme linked immunosorbent as say (ELISA) or rapid ELISA procedure detected immunoglobulin G (IgG) anti-F 1 fraction in human serum employing this derivative. The appropriate condit ions for carrying out the test were established as an antigen concentration of 2 mug/PANI-dacron disk, peroxidase labeled goat anti-human IgG conjugat e diluted 4000 times, and a serum dilution of 1:100. The PANI-dacron disks showed greater antigen retention than conventional poly(vinyl chloride) pla tes and less antibody unspecific adsorption. (C) 2001 John Wiley & Sons, In c. J Biomed Mater Res 56: 257-260, 2001.