Nerve growth factor and brain-derived neurotrophic factor mRNAs are regulated in distinct cell populations of rat heart after ischaemia and reperfusion
Jo. Hiltunen et al., Nerve growth factor and brain-derived neurotrophic factor mRNAs are regulated in distinct cell populations of rat heart after ischaemia and reperfusion, J PATHOLOGY, 194(2), 2001, pp. 247-253
Citations number
38
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Neurotrophins play a crucial role in the development of the peripheral nerv
ous system and their mRNAs are often regulated after several types of tissu
e injury. This study has investigated the regulation of nerve growth factor
(NGF), brain-derived neurotrophic factor (BDNF), and neurotrophin-3 (NT-3)
mRNAs 30 min after myocardial ischaemia followed by reperfusion, by northe
rn blotting, and in situ hybridization in a rat model. Between 2 and 120 h
of reperfusion, Ngf. mRNA levels showed two- to four-fold up-regulation com
pared with sham-operated hearts. Scattered;Ngf-expressing cells, probably p
ericytes, were detected in the viable border zone of the myocardium in clos
e association with capillaries, venules, and arterioles, In addition, diffu
se;Ngf expression was seen in the infarct area after 120 h of reperfusion,
Bdnf mRNA showed transient up-regulation after 2 and 5 h of reperfusion and
remained at control levels thereafter. Bdnf was expressed in the myocytes
of the viable border zone. Nt-3 expression showed no significant changes co
mpared with sham-operated hearts. These results suggest a role for NGF and/
or BDNF in the pathogenesis of reperfusion injury or in the alterations of
cardiac sensory and sympathetic neuronal function after myocardial ischaemi
a and reperfusion. Copyright (C) 2001 John Wiley & Sons, Ltd.