Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH center dot (2,2-diphenyl-1-picrylhydrazyl) free radical assay

Citation
L. Parejo et al., Evaluation of scavenging activity assessed by Co(II)/EDTA-induced luminol chemiluminescence and DPPH center dot (2,2-diphenyl-1-picrylhydrazyl) free radical assay, J PHARM TOX, 44(3), 2000, pp. 507-512
Citations number
26
Categorie Soggetti
Pharmacology & Toxicology
Journal title
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
ISSN journal
10568719 → ACNP
Volume
44
Issue
3
Year of publication
2000
Pages
507 - 512
Database
ISI
SICI code
1056-8719(200011/12)44:3<507:EOSAAB>2.0.ZU;2-B
Abstract
The scavenging activities of three standard antioxidants, quercetin, ascorb ic acid, and trolox, were evaluated by Co(II)/ethylenediaminetetraacetic ac id (EDTA)-induced luminol chemiluminescence and the 2,2-diphenyl-1-picrylhy drazyl (DPPH) free radical assay. Therefore, the aim of this study was to c haracterise an enzyme-free and time-independent chemiluminescence method fo r the assessment of the scavenging profile of compounds in a cell-free syst em using the Co(II)/EDTA-luminol-peroxide system. These results showed that the three standards were efficient and effective in inhibiting both Co(II) /EDTA-induced luminol chemiluminescence and the free radical DPPH. For all the data obtained in this work, the scavenging activity for the standards t ested decreased in the following order: quercetin>trolox> ascorbic acid. Th e present study has applied a simple and precise procedure for the study of hydroxyl radical scavenging activity by Co(II))/EDTA-induced luminol chemi luminescence, and this was assessed by DPPH free radical scavenging. (C) 20 01 Elsevier Science Inc. All rights reserved.