Analysis of oligosaccharide conformation by NMR spectroscopy utilizing H-1,H-1 and H-1,C-13 residual dipolar couplings in a dilute liquid crystallinephase

Citation
K. Lycknert et al., Analysis of oligosaccharide conformation by NMR spectroscopy utilizing H-1,H-1 and H-1,C-13 residual dipolar couplings in a dilute liquid crystallinephase, J PHYS CH A, 105(21), 2001, pp. 5119-5122
Citations number
31
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF PHYSICAL CHEMISTRY A
ISSN journal
10895639 → ACNP
Volume
105
Issue
21
Year of publication
2001
Pages
5119 - 5122
Database
ISI
SICI code
1089-5639(20010531)105:21<5119:AOOCBN>2.0.ZU;2-Q
Abstract
A novel procedure for determining H-1,H-1 NMR dipolar couplings in a dilute liquid crystalline medium based on H-1,H-1 COSY spectroscopy and a J-doubl ing procedure is presented. Subsequent conformational analysis of the centr al region of a partially deuterated decasaccharide employing H-1,H-1 and H- 1,C-13 dipolar couplings, H-1.H-1 nuclear Overhauser effect spectroscopy, a nd computer modeling is described. In particular, the use of H-1,H-1 dipola r couplings is of paramount importance in deriving a three-dimensional stru cture of the decasaccharide.