Fumonisins were produced by six strains of Fusarium moniliforme and Fusariu
m proliferatum on inoculated rice culture. For study of fumonisins of the B
series (B1, B2, B3, and B4) a quick and efficient two-step reversed-phase
OPLC method was developed in which the first step serves for cleaning of th
e samples. Measurements were performed with the automatic OPLC instrument.
Results showed that one isolate of F. moniliforme and one of F. proliferatu
m produced all the fumonisins investigated - the other isolates produced no
fumonisins, This work is the first report of the separation of all the fum
onisins of the B series by use of a planar layer liquid chromatographic tec
hnique.