Separation of fumonisins by OPLC

Fumonisins were produced by six strains of Fusarium moniliforme and Fusariu m proliferatum on inoculated rice culture. For study of fumonisins of the B series (B1, B2, B3, and B4) a quick and efficient two-step reversed-phase OPLC method was developed in which the first step serves for cleaning of th e samples. Measurements were performed with the automatic OPLC instrument. Results showed that one isolate of F. moniliforme and one of F. proliferatu m produced all the fumonisins investigated - the other isolates produced no fumonisins, This work is the first report of the separation of all the fum onisins of the B series by use of a planar layer liquid chromatographic tec hnique.