Isotype specific immune responses in murine experimental toxocariasis

In this work, a murine experimental model of toxocariasis has been develope d in BALB/c, C57BL/10 and C3H murine strains orally inoculated with 4,000 T oxocara canis embryonated eggs, in order to investigate the isotype-specifi c immune responses against excretory-secretory antigens from larvae. T. can is specific IgG+M, IgM, IgG, IgA, IgG1, IgG2a and IgG3 were tested bq ELISA . The dynamics of the specific immunoglobulins (IgG+IgM) production showed a contrasting profile regarding the murine strain. Conversely to the result s obtained with the IgM isotype, the IgG antibody class showed en similar p atterns to those obtained with IgG+IgM antibodies. only in the case of the BALB/c strain, being different and much higher than the obtained with IgG+I gM antibodies, When the C3H murine strain was used. The antibodies IgG+IgM tested in BALB/c and C57BL/10 were both of the IgM and IgG isotypes. Conver sely. in the C3H strain only IgG specific antibody levels were detected. Th e IgG1 subclass responses showed a similar profile in the three murine stra ins studied with high values in BALB/c, as in the case of the IgG responses .