Candida albicans cell wall lytic enzyme produced by Streptomyces thermodiastaticus

The production of lytic enzyme by Streptomyces thermodiastaticus was found to be affected by some growth conditions and nutritional factors. The highe st enzyme production was obtained after 18 h of incubation at pH 5.5 and at 50 degreesC. The carbon source influenced the lytic enzyme production. A h igher enzyme yield was obtained when Candida albicans cell wall (1 g/100 mi ) was used as the sole carbon source. NaNO3 at 0.1 g/100 mi was the best ni trogen source for enzyme production. From all phosphorous sources, microele ments, and growth factors tested, KH,PO, (1 g/l), ZnSO4(1 mg/l) and Tween 8 0 (0.1%), respectively, were found to favour the highest production of lyri c enzymes by S. thermodiastaticus. The lytic enzymes mainly produced chitin olytic and proteolytic activities.