Characterisation of neuropeptide Y receptor subtypes by synthetic NPY analogues and by anti-receptor antibodies

Neuropeptide Y (NPY), a 36-mer neuromodulator, binds to the receptors Y-1, Y-2, Y-4 and Y-5 with nanomolar affinity. They all belong to the rhodopsin- like G-protein coupled, seven transmembrane helix spanning receptors. In th is study, Ala-substituted and centrally truncated NPY analogues were compar ed with respect to affinity to the Y-receptors. Furthermore, antibodies aga inst the second (E2) and the third (E3) extracellular loop of NPY Y-1-, Y-2 - and Y-5-receptor subtypes were raised and affinity to intact cells was te sted by immunofluorescence assays. Both methods were applied in order to re ceive subtype selective tools and to characterise ligand binding. The analo gues [A(13)]-pNPY and [A(27)]-pNPY showed subtype selectivity for the Yz-re ceptor. Sera against the E2 loop of the Y-1-receptor and against the E2 loo p of the Y-2-receptor were subtype selective. Two antibodies against the Y- 5 E2 and E3 loop recognised the Y-5- and Y-2-receptor subtypes. In combinat ion, these sera are able to distinguish between the Y-1-, Y-2-, and Ys-rece ptor subtypes. The analogues and antibodies represent valuable tools to dis tinguish NPY receptors on membranes and intact cells.