Characterization of an ocular photopigment capable of driving pupillary constriction in mice

This work demonstrates that transgenic mice lacking both rod and cone photo receptors (rd/rd cl) retain a pupillary light reflex (PLR) that does not re ly on local iris photoreceptors. These data, combined with previous reports that rodless and coneless mice show circadian and pineal responses to ligh t, suggest that multiple non-image-forming light responses use non-rod, non -cone ocular photoreceptors in mice. An action spectrum for the PLR in rd/r d cl mice demonstrates that over the range 420-625 nm, this response is dri ven by a single opsin/vitamin A-based photopigment with peak sensitivity ar ound 479 nm (opsin photopigment/OP479). These data represent the first func tional characterization of a non-rod, non-cone photoreceptive system in the mammalian CNS.