Aj. Hao et al., Induction of cytokine expression in the brain macrophages/amoeboid microglia of the fetal rat exposed to a teratogen, NEUROREPORT, 12(7), 2001, pp. 1391-1397
Using in situ hybridization, RT-PCR, lectin histochemistry and immunohistoc
hemistry, this study examined the time course expression and cellular local
ization of various cytokines including tumor necrosis factor-alpha (TNF-alp
ha), interleukins (IL-1, IL-6) and transforming growth factor-beta (TGF-bet
a) in fetal rat brain after a maternal injection of the teratogen cyclophos
phamide (CP). Eight hours after CP injection, there was a marked increase i
n brain macrophages (BM)/amoeboid microglia (AM) in different areas of the
fetal brain as determined by lectin histochemistry. Concomitant to this was
the induction in mRNA level of TNF-alpha, which was progressively increase
d with time. TGF-beta mRNA was undetectable until 24 h had elapsed. Express
ion of IL-1 and IL-6 was undetectable at all stages. In situ hybridization
combined with immunohistochemistry has shown the localization of TNF-alpha
in PM/AM and neurons. Present results suggest that both TNF-alpha and TGF-b
eta are involved in the progression of neural damage in the fetal brain ind
uced by the teratogen. NeuroReport 12:1391-1397 (C) 2001 Lippincott William
s & Wilkins.