Role of ATP hydrolysis by UvrA and UvrB during nucleotide excision repair

Nucleotide excision repair in eubacteria is a process that repairs DNA dama ges by the removal of a 12-13-mer oligonucleotide containing the lesion. Re cognition and cleavage of the damaged DNA is a multistep ATP-dependent reac tion that requires the UvrA, UvrB and UvrC proteins. Both UvrA and UvrB are ATPases, with UvrA having two ATP binding sites which have the characteris tic signature of the family of ABC proteins and UvrB having one ATP binding site that is structurally related to that of helicases. (C) 2001 Editions scientifiques et medicales Elsevier SAS.