Detection of viral DNA and E4 protein in basal keratinocytes of experimental canine oral papillomavirus lesions

Citation
Pk. Nicholls et al., Detection of viral DNA and E4 protein in basal keratinocytes of experimental canine oral papillomavirus lesions, VIROLOGY, 284(1), 2001, pp. 82-98
Citations number
61
Categorie Soggetti
Microbiology
Journal title
VIROLOGY
ISSN journal
00426822 → ACNP
Volume
284
Issue
1
Year of publication
2001
Pages
82 - 98
Database
ISI
SICI code
0042-6822(20010525)284:1<82:DOVDAE>2.0.ZU;2-E
Abstract
We studied experimental canine oral papillomavirus (COPV) infection by in s itu hybridization and immunohistochemistry of weekly biopsies. After 4 week s, viral DNA in rete ridges suggested a keratinocyte stem cell target. Abun dant viral DNA was seen in E4-positive cells only. E4 was predominantly cyt oplasmic but also nuclear, being concentrated in the nucleoli during wart f ormation, infected cells spread laterally along the basal layer and into th e parabasal layers, accompanied by E7 transcription and increased mitoses. Most of the lower epithelium was positive for viral DNA, but, in mature war ts, higher levels of E4 expression and genome amplification occurred in onl y sporadic superficial cells. L1 expression was late and in only a subset o f E4-positive cells. During regression, viral DNA was less abundant in deep epithelial layers, suggesting downregulation of replication prior to repla cement of infected cells from beneath. Detection of viral DNA in post-regre ssion tissue indicated latent infection. (C) 2001 Academic Press.