THE N-GLYCOSYLATION SITES OF SOYBEAN SEED COAT PEROXIDASE

Tryptic digestion of ape-soybean peroxidase (apo-SBP), with and withou t acetamidation, chromatographic separation of the tryptic fragments a nd MALDI-TOF analysis of the major components, both before and after d igestion with glycopeptidase A, demonstrated the presence of six carbo hydrate groups on five peptides, Five of the glycopeptides can be mapp ed with confidence to the peptides containing Asn16, Asn90, Asn104, As n169, and Asn174, The sixth N-glycosylation site is not known and does not appear to be Asn145, It may be present on the N-terminus of SBP, which has not been sequenced.