Fluorescence resonance energy transfer analysis of CCR2-V64I and SDF1-3 ' A polymorphisms: Prevalence in southern Spain HIV type 1(+) cohort and noninfected population

The relationship between host genotype and AIDS, as well as the different g enotype frequencies observed in different populations, have become importan t topics in HIV research. Therefore, the development of methods that provid e faster and reliable results may contribute to further development and kno wledge of those topics. We present the results of genotyping SDF1-3'A and C CR2-V64I in 440 HIV-1-infected people and 100 noninfected controls from sou thern Spain, using a novel method based on real-time PCR with LightCycler t echnology and fluorescence resonance energy transfer. Frequencies obtained were 23.8% for SDF1-3'A and 9.5% for CCR2-V64I for both HIV+ cohort and gen eral population. Both polymorphisms are in accordance with the Hardy-Weinbe rg equilibrium law and no differences between patients and controls have be en observed.