RAPID ANALYSIS OF MALATHION IN BLOOD USING HEAD SPACE SOLID-PHASE MICROEXTRACTION AND SELECTED-ION MONITORING

A simple and rapid method for analysis of malathion in blood was devel oped using head space-solid phase microextraction (HS-SPME) and gas ch romatography mass spectrometry/ electron impact ionization-selected io n monitoring (GC-MS/EI-SIM). A vial containing a blood sample, ammoniu m sulphate, sulphuric acid and fenitrothion as an internal standard, w as heated at 90 degrees C for 15 min. The extraction fiber of the SPME was exposed for 5 min in the head space of the vial. The compounds ab sorbed on the fiber were detached by exposing the fibre in the injecti on port of GC-MS. A straight calibration curve was obtained between ma lathion concentrations of 2.5 to 50.0 mu g g(-1) in blood. No interfer ing substances were found, and the time for analysis was 40 min for on e sample. (C) 1997 Elsevier Science Ireland Ltd.