Green tea polyphenols: Novel irreversible inhibitors of dopa decarboxylase

The green tea gallocatechins, (-)-epigallocatechin-3-O-gallate (EGCG), and (-)-epigallocatechin (EGC) were found to be inhibitors of Dopa decarboxylas e (DDC). EG;CG; and EGC inactivate the enzyme in both a time- and concentra tion-dependent manner and exhibit saturation of the rate of inactivation at high concentrations, with efficiency of inactivation values (k(inact)/K-i) of 868 and 1511 M-1 min(-1), respectively. In contrast, gallic acid behave s as a weak inhibitor of DDC. Protection against inactivation by EGCG; and EG;C was observed in the presence of the active site-directed inhibitor D-D opa. Either EGCG or EGC induce changes in the absorbance and CD bands of th e visible spectrum of enzyme-bound PLP. Taken together, these findings indi cate the active site nature of the interaction of DDC with both polyphenols . On the basis of the properties of the EGCG-inactivated enzyme, it can be suggested that inactivation could be ascribed to a covalent modification of not yet identified residue(s) of the active site of DDC. (C) 2001 Academic Press.