Efficient synthesis of O-linked glycopeptide by a transglycosylation usingendo alpha-N-acetylgalactosaminidase from Streptomyces sp.

Authors
Ajisaka, K Miyasato, M Ishii-Karakasa, I
Citation
K. Ajisaka et al., Efficient synthesis of O-linked glycopeptide by a transglycosylation usingendo alpha-N-acetylgalactosaminidase from Streptomyces sp., BIOS BIOT B, 65(5), 2001, pp. 1240-1243
Citations number
17
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
65
Issue
5
Year of publication
2001
Pages
1240 - 1243
Database
ISI
SICI code
0916-8451(200105)65:5<1240:ESOOGB>2.0.ZU;2-5
Abstract
GaI beta-(1 -->3)-Ga1NAc-linked hexapeptide was synthesized by a transglyco sylation using GaI beta-(1 -->3)-Gal-NAc beta -pNP as a donor and a serine- containing hexapeptide as an acceptor using endo GaINAc-ase from Streptomyc es sp.. The GaI beta-(1 -->3)-GaINAc residue was transferred to the hydroxy l group of the serine residue of the peptide. The total yield of the glycop eptide via this process,vas better than that of the chemoenzymatic method. This profess was confirmed to be a versatile method for the synthesis of O- linked glycopeptides.