J. Masarova et al., Optimization of dextran and mannan dialdehydes preparation and examinationof their biospecific interaction with Concanavalin A, CHEM PAP-CH, 55(2), 2001, pp. 130-135
The periodate oxidation of dextran and mannan was optimized to prepare acti
vated polysaccharides (containing aldehyde groups) suitable for conjugation
with proteins. The mild reaction conditions to achieve available degree of
oxidation, while the depolymerization was prevented, were estimated. The p
roducts containing up to 16 aldehyde groups per dextran molecule and up to
28 aldehyde groups per mannan molecule were obtained, while a low decrease
of M-r of both polysaccharide chains, from 18 400 to 17 000 of dextran and
from 55 000 to 49 500 of mannan, respectively, was observed.
Using the precipitation method the interaction of Concanavalin A with both.
original as well as modified, subsequently reduced, dialdehyde polysacchar
ides was investigated. Only the precipitation of mannan and its modified fo
rm with Concanavalin A was observed. The oxidation of mannan up to 20 aldeh
yde groups per mannan molecule caused only a very little decrease of affini
ty of prepared samples to Concanavalin A.