The objective of this work is to give biologists the ability to visualize m
ulticolor laser confocal microscope (MLCM) data sets. We present the applic
ation of volume ray casting techniques to create fast and accurate visualiz
ation of the spatial structures in MLCM data sets. We provide various volum
e pre-processing routines for noise removal, smoothing and edge enhancement
. The tool has an interactive interface to assist the operator in classifyi
ng the volume using various transparency mappings. We have developed volume
rendering using volumetric ray casting based upon a perspective camera mod
el. We illustrate an innovative way to simultaneously visualize LCM data se
t from three lasers using voxel level merging. A method to generate key fra
me animation sequence of an arbitrary fly through path is also included in
the visualization tool. (C) 2001 Elsevier Science Ltd. All rights reserved.