Development and characterization of common wheat-Thinopyrum intermedium translocation lines with resistance to barley yellow dwarf virus

We developed some wheat-Th. intermedium translocation lines, Yw642, Yw443 a nd Yw243, etc., showing good BYDV resistance from L1 by induced homoeologou s pairing using CS ph mutant. Characterization of these wheat lines was car ried out by GISH and RFLP analysis. The results of GISH showed that the lin es, YWw42, Yw443 and Yw243, etc., are homozygous wheat- Th. intermedium tra nslocation lines, in which the chromosome segments of Th. intermedium were transferred to the distal end of a pair of wheat chromosomes. RFLP analysis indicated that the translocation chromosome of the wheat lines is T7DS.7DL -7XL. The breakpoint of the translocation is located on the distal end of 7 DL, between Xpsr965 and Xpsr680 about 90-99 cm from the centromere. The BYD V gene is located on the distal end of 7XL around Xpsr680, Xpsr687 and Xwg3 80. The RFLP markers of psr680, psr687 and wg380 were cosegregated with the BYDV resistance respectively and could be used for molecular assisted sele ction (MAS) in wheat breeding program for BYDV resistance.