Pro- and anti-inflammatory cytokine gene expression in rat sciatic nerve chronic constriction injury model of neuropathic pain

Cytokines may be pathophysiologically involved in hyperalgesia, Uncertainty exists about the types of cytokines and their site of action. To study the role of key pro- and anti-inflammatory cytokines in a chronic constriction model of neuropathic pain, mRNA expression of TNF, IL-1 beta, IL-6, and IL -10 was quantified using competitive RT-PCR. Each cytokine mRNA in rat scia tic nerve was examined at days 3, 7, 14, and 45 after chronic constriction injury (CCI), We also undertook behavioral testing of these rats. Thermal w arming and touch thresholds were significantly reduced at days 3, 7, and 14 in the CCI group, compared with the sham-operated group. Cytokine gene exp ression in sciatic nerve was significantly increased at day 7 for IL-1 beta and IL-6 and at day 14 for TNF. Expression of IL-10 underwent a gradual an d progressive increase, reaching statistical significance at day 45. (C) 20 01 Academic Press.