Catalytic activity of ADAM28

ADAMs are membrane-anchored glycoproteins containing a disintegrin and meta lloprotease domain that have important roles in fertilization, development, and diseases such as Alzheimer's dementia, Here we present the first evide nce for catalytic activity of ADAM28, a protein that is highly expressed in the epididymis and lymphocytes. Recombinant ADAM28 cleaves myelin basic pr otein at two sites. The catalytic activity of ADAM28 is not sensitive to ti ssue inhibitors of metalloproteases 1 and 2, but can be abolished by a muta tion in the catalytic site. Catalytically active ADAM28 will be valuable fo r further studies of its role in sperm maturation and lymphocyte function, (C) 2001 Published by Elsevier Science B,V, on behalf of the Federation of European Biochemical Societies.x