Applications of the telomerase assay in peritoneal washing fluids

Objective. The aim of this study was to detect telomerase activity in perit oneal ascites and to assess whether it can be used as an assistant tool for the early detection of ovarian cancer. Methods. Telomerase activity was measured by TRAP assay in 47 patients with ovarian malignancies and 50 patients with benign uterine leiomyomas (contr ol group). Results. All 26 peritoneal washing cytology positive cases were telomerase positive. Of the 21 peritoneal washing cytology negative cases, 3 were telo merase positive. When these 3 were reevaluated for peritoneal cytology, mal ignant ascitis was identified in 1. All telomerase negative cases were nega tive for peritoneal washing cytology. The sensitivity and specificity of pe ritoneal cytology and telomerase testing in correlation with true malignant cells were 96 (26/27) and 100% (20/20) versus 100 (27/27) and 90% (18/20), respectively. The false negative rate of peritoneal cytology was 4.7% (1/2 1). The false positive rate of the telomerase test in relation to malignant ascites was 6.9% (2/29). Conclusion. Our preliminary results reveal a high sensitivity and specifici ty of both telomerase testing and conventional cytology in peritoneal fluid s. Our data suggest that the telomerase test in peritoneal fluids can be us ed as an adjuvant to cytopathological methods in the diagnosis of malignant peritoneal ascites, particularly in cases of negative cytology. In these c ases, a review of peritoneal histocytology is advised. (C) 2001 Academic Pr ess.