K. Katayama et al., Method of investigation in rhizobacterial flora of crops and application case by using Biolog's identification system, JPN J CROP, 69(2), 2000, pp. 235-241
Fifty strains of rhizobacteria were isolated from roots of crops in rotatio
n and monocropping plots of soybeans and sweet potatos one month before har
vest, and they were identified by using Biolog's identification system duri
ng the period from 1994 to 1997. The strains of 38.3% of the rhizobacteria
isolates were identified, and the others were on the process of classificat
ion or unidentified. It was assumed that the relative high percentage in un
identification was attributed to insufficient database related to environme
ntal bacteria. If this database were thoroughly improved, however, this sys
tem would be useful for dynamic analyses of rhizobacterial flora in terms o
f genus or species level, since a large amount of strains of rhizobacteria
of crops could be smoothly identified with this system. The effects of rota
tion and monocropping systems on the rhizobacterial flora of crops were eva
luated by a diversity index using this system. The diversification of the r
hizobacterial nora was not improved in the monocropping plot of soybeans co
mpared with rotation plots of soybeans and sweet potatos and the monocroppi
ng plot of sweet potatos.