Speciation of zinc in low molecular weight proteins of breast milk and infant formulas by size exclusion chromatography/flame atomic absorption spectroscopy
P. Bermejo et al., Speciation of zinc in low molecular weight proteins of breast milk and infant formulas by size exclusion chromatography/flame atomic absorption spectroscopy, J AOAC INT, 84(3), 2001, pp. 847-852
Size exclusion chromatography (SEC) and flame atomic absorption spectroscop
y (FAAS) were used for the separation of metal-containing species in milk w
hey. After milk ultracentrifugation, the sample was injected into a TSK-Gel
G2000 glass column and eluted with 0.2M NH4NO3-NH3, pH 6.7, Low molecular
weight proteins were fractionated, and the fractions were characterized by
molecular weight. Zinc distributions were obtained by FAAS using a high per
formance nebulizer, The method was very sensitive (limit of detection = 2.6
x 10(-3) mug/mL; limit of quantitation = 8.9 x 10(-3) mu /mL) and precise
(RSDs less than or equal to 10%), This method was applied to the determinat
ion of Zn in binding compounds in breast milk whey and in commercial cow's
milk-based formulas, Distribution patterns were different. The presence of
Zn in most fractions in breast milk was most significant, whereas in infant
formulas Zn was detected only in fractions of molecular weight <5 kDa and
in the highest molecular weight peak.