I. Saadoun et al., Assessment of toxic potential of local Jordanian Bacillus thuringiensis strains on Drosophila melanogaster and Culex sp (Diptera), J APPL MICR, 90(6), 2001, pp. 866-872
Aims: To assess the toxic potential of different local Jordanian Bacillus t
huringiensis isolates on larvae of Drosophila melanogaster and Culex sp.
Methods and Results: Scanning electron microscopy revealed the presence of
spherical, bi-pyramidal, and bi-pyramidal and cuboidal parasporal bodies pr
oduced by the toxic isolates. Spherical inclusions dominated. The toxicity
of the isolates to the two insects, determined using 24-well plates or vial
s, indicated that the 50% lethal concentration (LC50) of the bacterial susp
ension for D. melanogaster and Culex sp, larvae varied from 4.60 to 8.65 an
d from 5.30 to 6.74, respectively.
Conclusions: Comparison of the LC50 values of isolate 8(2) with those of th
e reference strain B. t. israelensis showed that this isolate has a higher
toxicity potential.
Significance and Impact of the Study: Some local Jordanian B. thuringiensis
isolates exhibit toxic potential that could be used to control some import
ant pests, and could replace chemical pesticides.