CD95 signaling via ceramide-rich membrane rafts

Citation
H. Grassme et al., CD95 signaling via ceramide-rich membrane rafts, J BIOL CHEM, 276(23), 2001, pp. 20589-20596
Citations number
35
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
276
Issue
23
Year of publication
2001
Pages
20589 - 20596
Database
ISI
SICI code
0021-9258(20010608)276:23<20589:CSVCMR>2.0.ZU;2-K
Abstract
Clustering seems to be employed by many receptors for transmembrane signali ng. Here, we show that acid sphingomyelinase (ASM)-released ceramide is ess ential for clustering of CD95, In vitro and in vivo, extracellularly orient ated ceramide, released upon CD95-triggered translocation of ASM to the pla sma membrane outer surface, enabled clustering of CD95 in sphingo lipid-ric h membrane rafts and apoptosis induction. Whereas ASM deficiency, destructi on of rafts, or neutralization of surface ceramide prevented CD95 clusterin g and apoptosis, natural ceramide only rescued ASM-deficient cells. The dat a suggest CD95-mediated clustering by ceramide is prerequisite for signalin g and death.