The usefulness of a nested PCR assay for detection of Aspergillus so. DNA w
as evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test
was accurate both to diagnose culture-negative BAL fluid specimens from pa
tients with invasive pulmonary aspergillosis and to confirm culture-positiv
e samples. However, if did not differentiate between infection and coloniza
tion.