Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals

Authors
Wagenaar, JA van Bergen, MAP Newell, DG Grogono-Thomas, R Duim, B
Citation
Ja. Wagenaar et al., Comparative study using amplified fragment length polymorphism fingerprinting, PCR genotyping, and phenotyping to differentiate Campylobacter fetus strains isolated from animals, J CLIN MICR, 39(6), 2001, pp. 2283-2286
Citations number
18
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
6
Year of publication
2001
Pages
2283 - 2286
Database
ISI
SICI code
0095-1137(200106)39:6<2283:CSUAFL>2.0.ZU;2-6
Abstract
A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to th e subspecies level and genotypically typed by PCR and amplified fragment le ngth polymorphism (AFLP) analysis. Phenotypic subspecies determination meth ods were unreliable. Genotyping of the strains by PCR and AFLP showed a cle ar discrimination between the two subspecies.