The vase life and floret opening of cut tuberose inflorescences were signif
icantly decreased by cold storage. The ideal storage temperature was found
to be 0 degreesC for short durations because even storage at 2 degreesC for
only 3d significantly decreased floret opening and the vase life of stored
inflorescences. There was no significant difference between "wet" storage
in a preservative solution (250 ppm 8-hydroxyquinoline citrate, 2% sucrose)
and "dry" storage (spikes wrapped in polythene film to reduce water loss).
Pre-storage pulsing with a 20% sucrose solution (containing HQC) significa
ntly improved the vase life and opening of cold-stored spikes. The vase lif
e and floret opening of spikes treated in this way were equal, after 6 d of
storage at 2 degreesC, to those of fresh cut inflorescences. The end of th
e vase life of cut tuberose spikes coincided with the time taken to return
to their initial fresh weight. Cold storage resulted in a pronounced increa
se in ethylene production by the florets, particularly by immature buds. Et
hylene treatment of fresh cut tuberose spikes reduced floret opening, but e
thylene induced by cold storage did not appear to be the cause of reduced f
loret opening. Pretreatment of spikes with STS eliminated the effects of ex
ogenous ethylene on fresh spikes, but had no effect on the reduced vase lif
e of cold-stored flowers and substantially increased ethylene production by
their florets. Ethylene production by florets of sucrose-pulsed spikes was
similar to that of the controls. It appears that the shortened vase life o
f cold-stored tuberose is not due to the induction of ethylene biosynthesis
.