V. Deneys et al., Reference values for peripheral blood B-lymphocyte subpopulations: a basisfor multiparametric immunophenotyping of abnormal lymphocytes, J IMMUNOL M, 253(1-2), 2001, pp. 23-36
Background and objectives: Immunophenotyping has become a useful tool for t
he differential diagnosis of chronic B-cell lymphoproliferative disorders.
The aim of this work was to determine reference values of normal B-cell sub
populations. Material and methods: Blood samples from 38 healthy volunteers
were analyzed by multidimensional flow cytometry, using a panel of directl
y conjugated antibodies. Results were expressed as percent of positive B ce
lls and as median fluorescence intensity, an indirect assessment of the exp
ression level. Results: CD20, CD22, CD24, CD40, CD79a, CD79b, FMC7, CD11a,
CD18, CD44 were positive in the whole B cell population, whereas CD10, CD86
, CD103, CD154 and Fast were almost absent from the B-lymphocyte population
. 75% were IgD positive. The kappa/lambda ratio was 1.5. CD5, CD23, CD25, C
D38, CD43, CD54, CD62L, CD80 and CD95 were positive in different B-cell sub
populations. The utility of all these markers in the differential diagnosis
of chronic B-cell lymphoproliferative disorders is discussed. Conclusion:
In order to interpret a pathological immunophenotype, it is necessary to re
fer to quantitative and qualitative values of normal B-cell subpopulations.
(C) 2001 Elsevier Science B.V. All rights reserved.