Determination of ambroxol hydrochloride by HPLC

A rapid, specific, and reliable technique has been developed for the determ ination of ambroxol. hydrochloride in solution by high performance liquid c hromatography. To do so, reverse phase liquid chromatography was employed, using methanol-0.01 M diammonium phosphate buffer, pH=6, (70:30 v/v) and a detector wavelength of 247 nm, the concentration range employed being 90-12 0 mug/mL. The technique was seen to have excellent linearity, accuracy, and intra- an d inter-day precision for the concentration range studied, both when the re sponse was measured by peak areas and when measured by peak heights.