Jyk. Hsieh et al., High-throughput liquid chromatographic determination of rofecoxib in humanplasma using a fully automated on-line solid-phase extraction system, J LIQ CHR R, 24(6), 2001, pp. 799-812
A fully automated on-line solid-phase extraction method combined with high-
performance liquid chromatography-fluorescence detection for the determinat
ion of rofecoxib in human plasma is described. A Spark Holland PROSPEKT sys
tem was used to perform all the automated on-line sample extraction and qua
ntitation tasks, including sample loading, cartridge conditioning and washi
ng, analyte elution, and start of a data acquisition system. Under postcolu
mn ultraviolet-irradiation conditions, the analytes were photochemically co
nverted to highly fluorescent products and then monitored by a fluorescence
detector. The assay was validated in the concentration range of 0.5-100 ng
/mL using 0.25 mL of human plasma. The utility of this method was demonstra
ted by analysis of plasma samples from human subjects receiving oral doses
of rofecoxib.