Determination of clenbuterol HCl in human serum, pharmaceuticals, and in drug dissolution studies by RP-HPLC

A simple, reliable, and rapid RP-HPLC method has been developed for the det ermination of clenbuterol HCl in human serum and pharmaceuticals, in order to carry out drug dissolution studies for clenbuterol tablets. This compoun d is well separated on a C-18 column by using the mobile phase consisting o f a mixture of acetonitrile and ion-pair buffer (32:68; v/v) at a flow rate of 1.5 mL/min. Detection was carried out using a UV detector at 244 nm. ephedrine HCl was used as an internal standard. Minimum detection limit obtained was 3.78 ng/ mL. This method was applied, without any interferences from the excipients, for the determination of the drug in tablet formulation, human serum, and drug dissolution studies.