Hplc determination of vasicine and vasicinone in Adhatoda vasica with photo diode array detection

A high performance liquid chromatographic method for the determination of t he quinazoline alkaloids vasicine (1) and vasicinone (2) in Adhatoda vasica plant extract is reported. Peak purity and similarity of 1 and 2 have been studied using photodiode array detector (PDA). Effects of different solven ts have been studied for the extraction of 1 and 2 in A. vasica plant, and methanol was found to give the maximum extraction of compounds 1 and 2. The method is simple, sensitive, rapid, and reproducible for the quantitation of pharmacologically important alkaloids vasicine and vasicinone. The separ ation of 1 and 2 was performed with acetonitrile-phosphate buffer (pH maint ained to 3.9 using glacial acetic acid) (15:85) using a Hibar Merck make C- 18 column.