IMMOBILIZATION OF INVERTASE IN CONDUCTING POLYMER MATRICES

This paper reports a novel approach in the electrode immobilization of an enzyme, invertase, by electrochemical polymerization of pyrrole in the presence of enzyme. The polypyrrole/invertase and polyamide/polyp yrrole/invertase electrodes were constructed by the entrapment of enzy me in conducting matrices during electrochemical polymerization of pyr role. This study involves the preparation and characterization of poly pyrrole/invertase and polyamide/polypyrrole/invertase electrodes under conditions compatible with the enzyme. It demonstrates the effects of pH and temperature on the properties of enzyme electrode. Enzyme leak age tests were carried out during reuse number studies. The preparatio n of enzyme electrodes was done in two different electrolyte/solvent s ystems. The enzyme serves as a sucrose electrode and retains its activ ity for several months. (C) 1997 Elsevier Science Limited. All rights reserved.