The study of the doxorubicin adsorbed onto chemically modified silver films by surface-enhanced spectroscopy

This work presents some aspects of surface-enhanced spectroscopy applicatio n to the study of intermolecular interactions of antitumor drugs with micro environment in compound molecular systems. The surface-enhanced fluorescenc e (SEF) spectra and the surface-enhanced resonance Raman scattering (SERRS) spectra of doxorubicin (DOX) adsorbed on silver films (SF), annealed silve r films (ASF) and chemically modified ASF are exposed and compared with cor responding spectra of DOX in water solution and on silver sols. The existen ce of complicated dependencies for both (SERRS and SEF) components of surfa ce-enhanced secondary emission of DOX on ASF on optical and chemical proper ties of used films are discussed. Changes detectable in SERRS spectra of DO X at transition from solutions to sols, from sols to SF and further to ASF and chemically modified ASF are also discussed and considered as a result o f a change in number and/or activity of specific adsorption sites on silver surface. These spectral changes are very similar to those in resonance Ram an (RR) spectra of intercalated complex of DOX with DNA and in RR spectra o f aclacinomicin. The breakage of intramolecular hydrogen bonds and effects of changing of chromophore moiety local symmetry of DOX are considered. (C) 2001 Elsevier Science B.V. All rights reserved.