Hybrid vectors based on adeno-associated virus serotypes 2 and 5 for muscle-directed gene transfer

Authors
Hildinger, M Auricchio, A Gao, G Wang, L Chirmule, N Wilson, JM
Citation
M. Hildinger et al., Hybrid vectors based on adeno-associated virus serotypes 2 and 5 for muscle-directed gene transfer, J VIROLOGY, 75(13), 2001, pp. 6199-6203
Citations number
18
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
75
Issue
13
Year of publication
2001
Pages
6199 - 6203
Database
ISI
SICI code
0022-538X(200107)75:13<6199:HVBOAV>2.0.ZU;2-B
Abstract
Vectors based on hybrids consisting of adeno-associated virus types 2 (ITRs and Rep) and 5 (Cap) were evaluated fur muscle-directed gene transfer (cal led AAV215). Evaluation in immune-competent mice revealed greater transduct ion efficacy with AAV2/5 than with AAV2 and no cross-neutralization between AAV2/5 and AAV2. Interestingly, we saw no immunologic evidence of previous exposure to AAV5 capsids in a large population of healthy human subjects.