Organization and structural properties of langmuir films composed of conjugated polydiacetylene and phospholipids

Molecular assemblies containing phospholipids and conjugated polydiacetylen e lipids exhibit unique biockromatic properties and have attracted increasi ng interest in recent years as potential bio- and chemosensors. We present a detailed study of the properties of mixed films formed at the air-water i nterface, which consist of phospholipid molecules and diacetylene lipids. T he organization of the films has been characterized by surface pressure-are a isotherms. Application of atomic force microscopy, polarized optical micr oscopy, and W-vis spectroscopy provides further insight into the structures and interactions of the film components. The data indicate that the two co nstituents in the film are miscible at low surface pressure, while segregat ion of phospholipid and polymer domains occurs at higher surface pressures. The distribution and interactions between the diacetylene and phospholipid domains additionally depends on the molar fraction of phospholipid in the film. Characterization of the structural properties of the polydiacetylene domains in the films points to a formation of organized trilayer and multil ayer phases at high surface pressures and high diacetylene concentrations.