RESPONSES OF CARP HEPATOPANCREATIC 7-ETHOXYRESORUFIN-O-DEETHYLASE ANDGLUTATHIONE-DEPENDENT ENZYMES TO ORGANIC POLLUTANTS - A FIELD-STUDY

Modulations of hepatopancreatic activities of cytochrome P4501A (CYP1A ) and glutathione-dependent enzymes were investigated in carp collecte d in five ponds with different levels of contamination. The CYP1A-depe ndent 7-ethoxyresorufin-O-deethylase activity was markedly induced by polycyclic aromatic hydrocarbons present in the sediment at a total co ncentration of 0.9 mg/kg. Even a low organic contamination increased s ome of the glutathione-dependent enzymatic activities, namely cytosoli c glutathione reductase, glutathione S-transferase toward 1-chloro-2,4 -dinitrobenzene, ethacrynic acid and 1,2-epoxy-3-(p-nitrophenoxy)propa ne, and microsomal glutathione S-transferase. These parameters should be considered as potential tools for the biomonitoring of exposure to chemicals and/or impacts of exposure. An example of a multivariate clu ster and discriminant analysis of the obtained analytical and biochemi cal data proved to be very effective tools for the characterization of the level of contamination.