Purpose: The purpose of this study was to examine the test-retest reliabili
ty of maximal accumulated oxygen deficit (MAOD) measured at 110% and 120% o
f peak oxygen uptake (VO2) for cycling in seven untrained male and seven un
trained female subjects. Methods: After one familiarization trial, all subj
ects performed two MAOD tests at a power output corresponding to 110% and t
wo tests at 120% of VO2peak in random order. MAOD was calculated for each s
ubject as the difference between O-2 demand during exercise and the measure
d VO2. Results: The mean (+/- SEM) time to exhaustion for the group was not
significantly different between trial 1 (226 +/- 13 s) and trial 2 (223 +/
- 14 s) of the 110% test. Likewise, the difference in the time to exhaustio
n between trial 1 (158 +/- 11 s) and trial 2 (159 +/- 10 s) was not signifi
cant for the 120% test. The intraclass correlation coefficients for the tim
e to exhaustion were 0.95 for the 110% test and 0.98 for the 120% test. The
mean MAOD value obtained in trial 1 (2.62 +/- 0.17 L) was not significantl
y different from the mean value obtained in trial 2 (2.54 +/- 0.19 L)for th
e 110% test. Additionally, the mean values for the two trials did not diffe
r significantly for MAOD (2.64 +/- 0.21 L for trial 1 and 2.63 +/- 0.19 L f
or trial 2) in the 120% test. The intraclass correlation coefficients for M
AOD were 0.95 for the 110% test and 0.97 for the 120% test. All intraclass
correlation coefficients were significant at P < 0.001. Conclusions: When c
onducted under standardized conditions, the determination of MAOD for cycli
ng was highly repeatable at both 110% and 120% of VO2peak in untrained male
and female subjects.