A novel filamentous Bacillus sp., strain NAF001, forming endospores and budding cells

A novel filamentous bacterium, strain NAF001, was isolated from suspended w ater of a domestic wastewater treatment tank. It formed an extremely long f ilamentous trichome and produced endospores. It formed spore-like resting c ells (SLRCs) which were heat-resistant, SLRCs grew by budding to form short filaments resembling the gonidia of filamentous bacteria such as Leucothri x, This is the first report of a Bacillus species that exhibits budding gro wth. The filamentous form was neither restricted to any particular growth s tage nor dependent on cultural conditions, Phylogenetic analysis of the 16S rRNA gene revealed that this isolate was a member of the genus Bacillus, w ith no close relatives at the species level (sequence similarity < 95.3 %), Strain NAF001 thus probably belongs to a new and novel species of Bacillus .