Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 96 (DNA group 11)

Authors
Vinogradov, EV Pantophlet, R Brade, H Holst, O
Citation
Ev. Vinogradov et al., Structural and serological characterisation of the O-antigenic polysaccharide of the lipopolysaccharide from Acinetobacter strain 96 (DNA group 11), J ENDOTOX R, 7(2), 2001, pp. 113-118
Citations number
22
Categorie Soggetti
Immunology
Journal title
JOURNAL OF ENDOTOXIN RESEARCH
ISSN journal
09680519 → ACNP
Volume
7
Issue
2
Year of publication
2001
Pages
113 - 118
Database
ISI
SICI code
0968-0519(2001)7:2<113:SASCOT>2.0.ZU;2-T
Abstract
A polysaccharide containing D-Manp, L-Fucp (6-deoxygalactopyranose, fucose) and D-GlcpNac was isolated by mild acid hydrolysis, followed by gel-permea tion chromatography, from the lipopolysaccharide derived from Acinetobacter strain 96 (DNA group 11). The structure of the O-antigen was determined by compositional analysis and NMR spectroscopy of the polysaccharide as: [GRAPHICS] A monoclonal antibody obtained after immunization of mice with heat-killed bacteria of Acinetobacter strain 96 was shown to bind to the O-antigen and did not cross-react with any Acinetobacter O-antigen of known structure.