Composition of crocins and picrocrocin from Spanish saffron (Crocus sativus L.)

A method for simultaneous identification and quantification of crocins and picrocrocin by HPLC-DAD in aqueous extracts of saffron spice was used. The coefficients of variation obtained were lower than 10% for a majority of th e crocins and picrocrocin. These substances are not found as standard compo unds, for this reason it was necessary to obtain the response factor for ea ch compound by collecting the fractions of each chromatographic peak and by using molar absorpitivity. This method can be used nor only for crocins an d picrocrocin determination but also for adulteration detection of saffron with water-soluble colorants. The method was applied to 260 samples of Spanish saffron, which came from t he regions where saffron is an important crop, i.e., Castilla-La Mancha and Aragon. Spain is in the top group of saffron producers and Castilla-La Man cha is the largest saffron producing region in Spain. Four crocins and picr ocrocin were ranked and the values used for differentiation according to or igin. Only discriminant analysis could explain 100% of the variance by samp les and location. The major discriminant variable was trans-crocin 4.